Transcriptome sequencing analysis of sex-related genes and miRNAs in the gonads of Mytilus coruscus

Mytilus coruscus is a significant economic species in China’s eastern coastal areas. As dioecious species, it lacks secondary sexual characteristics, which makes difficult for selective breeding. However, limited research carried out on the genetic data regarding reproductive development and gender differentiation. In current study, de novo transcriptome sequencing analyses were used to detect gonad-specific genes miRNAs M. . By comparing testis ovary, 159,970 unigenes 300 obtained totally, of differentially expressed 9,566 25, respectively. Analysis qRT-PCR showed that cyp26a , dmrt4 foxl2 gdf9 17β-hsd14 sc6a9 zar1 zp4 highly ovary as compared testis, while sox2 lower expression ovary. Expression miR-750-3p, novel 1, miR-193 was higher than whereas miR-9-5p, miR-9-3p, miR-317, 124, miR-2d miR-263b Furthermore, analysis miR-317 by Targetscan MiRanda predicted target luciferase reporter performed confirm it. Our provides molecular basis understanding regulation. Further needed mechanism gonadal maturation differentiation